Composite

Part:BBa_K2680262

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G KanR

3G KanR; Kanamycin resistance gene flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

"Kanamycin resistance with two amber stop codons. The part was designed for the use in a positive selction plasmid to attain aa specific tRNA/synthetase, able to incorporate non canonical amino acids. The Kanamycin resistence is desined with two amber codons, resulting in a lacking resistence if an endogeneous amino acid ist incorporated."1

References

1Schlueter, L. (2017, October 29). Part:BBa_K2201414. Retrieved October 16, 2018, from https://parts.igem.org/wiki/index.php?title=Part:BBa_K2201414


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 879


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